Journal article
Analysis of Wnt signaling β-catenin spatial dynamics in HEK293T cells
CW Tan, BS Gardiner, Y Hirokawa, DW Smith, AW Burgess
BMC Systems Biology | Published : 2014
Abstract
Background: Wnt/β-catenin signaling is involved in different stages of mammalian development and implicated in various cancers (e.g. colorectal cancer). Recent experimental and computational studies have revealed characteristics of the pathway, however a cell-specific spatial perspective is lacking. In this study, a novel 3D confocal quantitation protocol is developed to acquire spatial (two cellular compartments: nucleus and cytosol-membrane) and temporal quantitative data on target protein (e.g. β-catenin) concentrations in Human Epithelial Kidney cells (HEK293T) during perturbation (with either cycloheximide or Wnt3A). Computational models of the Wnt pathway are constructed and interrogat..
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Awarded by National Health and Medical Research Council
Funding Acknowledgements
[ "The authors wish to thank Dr Maree Faux, Dr Meredith Layton and Mr Cameron Nowell for their thoughtful discussions and assistance rendered and Dr Bruno Catimel (Ludwig Institute for Cancer Research (Austin), Victoria, Australia) for providing binding affinity data used during this study. We thank the School of Engineering, University of Melbourne and the Ludwig Institute for Cancer Research, Melbourne-Parkville Branch (Victoria, Australia) for supporting CT.", "This work is also supported by National Health and Medical Research Council (NHMRC) Project Grants #542531, Program Grant #487922 and by funds from the Operational Infrastructure Support Program provided by the Victorian Government, Australia. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Additionally the manuscript is solely the responsibility of the institutions and individual authors and does not reflect the views of NHMRC." ]